1IXX

CRYSTAL STRUCTURE OF COAGULATION FACTORS IX/X-BINDING PROTEIN (IX/X-BP) FROM VENOM OF HABU SNAKE WITH A HETERODIMER OF C-TYPE LECTIN DOMAINS

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	288
Detector technology	IMAGE PLATE
Collection date	1996-05-28
Detector	RIGAKU
Spacegroup name	P 21 21 2
Unit cell lengths	172.200, 85.700, 65.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.500
R-factor	0.178
Rwork	0.178
R-free	0.22100
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	24.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.610
High resolution limit [Å]	2.500	2.500
Rmerge	0.053	0.229
Number of reflections	29883
<I/σ(I)>	39	6
Completeness [%]	87.0	75
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8		Mizuno, H., (1991) J. Mol. Biol., 220, 225. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	20 (mM)
2	1	reservoir		3 (mM)
3	1	reservoir	ammonium sulfate	60 (%sat)
4	1	drop	protein	10 (mg/ml)
5	1	drop	Tris-HCl	20 (mM)