1IXS
Structure of RuvB complexed with RuvA domain III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL24XU |
| Synchrotron site | SPring-8 |
| Beamline | BL24XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-11-13 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.836 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 102.060, 102.060, 137.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.200 |
| Rwork | 0.231 |
| R-free | 0.29300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hqc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.334 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.091 | 0.324 |
| Total number of observations | 235468 * | |
| Number of reflections | 12468 * | |
| <I/σ(I)> | 6.8 | 2.1 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | PEG 4000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RuvA domain III | 0.5 (mM) | |
| 10 | 1 | reservoir | PEG4000 | 10 (%(w/v)) | |
| 2 | 1 | drop | RuvB | 0.5 (mM) | |
| 3 | 1 | drop | AMPPNP | 1 (mM) | |
| 4 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
| 5 | 1 | drop | 0.1 (M) | ||
| 6 | 1 | drop | glycerol | 10 (%(w/v)) | |
| 7 | 1 | reservoir | Tris-HCl | 10 (mM) | pH8.0 |
| 8 | 1 | reservoir | 0.1 (M) | ||
| 9 | 1 | reservoir | glycerol | 10 (%(w/v)) |
