1IXR
RuvA-RuvB complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-01-24 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 61.581, 195.113, 246.244 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 3.300 |
Rwork | 0.268 |
R-free | 0.33500 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.720 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.059 | 0.474 |
Total number of observations | 139789 * | |
Number of reflections | 24710 | |
<I/σ(I)> | 28.9 | 3.5 |
Completeness [%] | 97.6 | 97.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | NaCl, ethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | RuvA | 0.6 (mM) | |
10 | 1 | reservoir | ethanol | 10 (%(w/v)) | |
2 | 1 | drop | RuvB | 0.8 (mM) | |
3 | 1 | drop | AMPPNP | 1 (mM) | |
4 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
5 | 1 | drop | 0.1 (M) | ||
6 | 1 | drop | glycerol | 10 (%(w/v)) | |
7 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
8 | 1 | reservoir | 1.5 (M) | ||
9 | 1 | reservoir | 0.1 (M) |