1IXR
RuvA-RuvB complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 61.581, 195.113, 246.244 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 3.300 |
| Rwork | 0.268 |
| R-free | 0.33500 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.720 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.059 | 0.474 |
| Total number of observations | 139789 * | |
| Number of reflections | 24710 | |
| <I/σ(I)> | 28.9 | 3.5 |
| Completeness [%] | 97.6 | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | NaCl, ethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RuvA | 0.6 (mM) | |
| 10 | 1 | reservoir | ethanol | 10 (%(w/v)) | |
| 2 | 1 | drop | RuvB | 0.8 (mM) | |
| 3 | 1 | drop | AMPPNP | 1 (mM) | |
| 4 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
| 5 | 1 | drop | 0.1 (M) | ||
| 6 | 1 | drop | glycerol | 10 (%(w/v)) | |
| 7 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 8 | 1 | reservoir | 1.5 (M) | ||
| 9 | 1 | reservoir | 0.1 (M) |
