1IX6
Aspartate Aminotransferase Active Site Mutant V39F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1998-12-05 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 156.107, 87.842, 80.507 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| Rwork | 0.205 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 * |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.068 * |
| Number of reflections | 26929 * |
| Completeness [%] | 95.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Ammonium Sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 10 (mM) | pH7.0 |
| 3 | 1 | drop | PLP | 0.010 (mM) | |
| 4 | 1 | drop | 0.3 (mM) | ||
| 5 | 1 | reservoir | potassium phosphate | 10 (mM) | pH7.0 |
| 6 | 1 | reservoir | ammonium sulfate | 40 (%sat) |
