1IWJ

Putidaredoxin-Binding Stablilizes an Active Conformer of Cytochrome P450cam in its Reduced State; Crystal Structure of Mutant(109K) Cytochrome P450cam

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	297
Detector technology	CCD
Collection date	2002-03-20
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	109.160, 104.330, 36.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.000
Rwork	0.168
R-free	0.22300
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.054	0.054
Number of reflections	27413
<I/σ(I)>	14	12.8
Completeness [%]	95.1	79.8
Redundancy	3.2	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6	293	ammonium sulfate, pH 6.0, SMALL TUBES, temperature 293K