1IWI

Putidaredoxin-Binding Stablilizes an Active Conformer of Cytochrome P450cam in its Reduced State; Crystal Structure of Cytochrome P450cam

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	297
Detector technology	CCD
Collection date	2001-10-20
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	109.160, 104.330, 36.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.000
R-factor	0.173 *
Rwork	0.177
R-free	0.22900 *
RMSD bond length	0.007 *
RMSD bond angle	1.134 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.032	0.061
Total number of observations	163126 *
Number of reflections	27250
<I/σ(I)>	13.2	11
Completeness [%]	95.1	79.8
Redundancy	3.9	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7 *	12-15 *	Poulos, T.L., (1982) J. Biol. Chem., 257, 10427. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1		50 (mM)
2	1	1		250 (mM)
3	1	1	dithiothreitol	50 (mM)
4	1	2	ammonium sulphate	66-70 (%sat)