1IW9

Crystal Structure of the M Intermediate of Bacteriorhodopsin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-11-18
Detector	RIGAKU
Wavelength(s)	0.708
Spacegroup name	P 6 2 2
Unit cell lengths	102.300, 102.300, 112.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.000 * - 2.500
R-factor	0.224
Rwork	0.224
R-free	0.28600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dze
RMSD bond length	0.007
RMSD bond angle	1.231 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.068 *	0.522 *
Number of reflections	10066 *
Completeness [%]	92.1 *	92.1 *
Redundancy	5.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	10 *	ammonium sulfate, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	purple membrane sheets	5 (mg/ml)
2	1	drop	octylthioglucoside	2.5 (mg/ml)
3	1	drop	ammonium sulfate	1.0 (M)
4	1	drop		0.16 (M)
5	1	drop	trehalose	12 (%(w/v))
6	1	drop		0.04 (%(w/v))
7	1	drop	sodium citrate	0.08 (M)	pH5.2
8	1	reservoir	ammonium sulfate	1.9-2.0 (M)