1IW8

Crystal Structure of a mutant of acid phosphatase from Escherichia blattae (G74D/I153T)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	288
Detector technology	IMAGE PLATE
Collection date	1997-12-03
Detector	FUJI
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	137.800, 168.200, 58.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.243
Rwork	0.243
R-free	0.28500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d2t
RMSD bond length	0.007
RMSD bond angle	1.310 *
Data reduction software	WEIS
Data scaling software	WEIS
Phasing software	AMoRE
Refinement software	CNX (2000.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.550
High resolution limit [Å]	2.500	2.500
Rmerge	0.084
Number of reflections	45889
Completeness [%]	97.5	91.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	20 *	PEG400, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris	20 (mM)	pH8.0
3	1	reservoir	PEG400	38 (%)
4	1	reservoir	Tris-HCl	20 (mM)	pH8.4