1IW7
Crystal structure of the RNA polymerase holoenzyme from Thermus thermophilus at 2.6A resolution
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45PX |
| Synchrotron site | SPring-8 |
| Beamline | BL45PX |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-01-25 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.02 |
| Spacegroup name | P 32 |
| Unit cell lengths | 236.350, 236.350, 249.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.600 |
| R-factor | 0.232 |
| Rwork | 0.228 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i6v |
| RMSD bond length | 0.020 * |
| RMSD bond angle | 2.260 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.600 | 2.600 * |
| Rmerge | 0.133 | 0.370 |
| Total number of observations | 1362723 * | |
| Number of reflections | 452740 * | |
| <I/σ(I)> | 5.4 | 2.5 |
| Completeness [%] | 94.6 * | 89.2 |
| Redundancy | 2.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | Mg formate, PEG400, Spermidine, TRIS HCl, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | magnesium formate | 13 (mM) | |
| 3 | 1 | reservoir | PEG400 | 5 (%) | |
| 4 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | MES | 20 (mM) | pH5.8 |
