1IVY
PHYSIOLOGICAL DIMER HPP PRECURSOR
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-03-24 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 115.040, 148.110, 80.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT AND TWO-FOLD AVERAGING |
| Starting model (for MR) | SERINE CARBOXYPEPTIDASE FROM WHEAT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.720 |
| Data reduction software | MOSFLM (5.2) |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.300 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.051 | 0.130 * |
| Total number of observations | 436709 * | |
| Number of reflections | 67740 | |
| <I/σ(I)> | 9.8 | 5.7 |
| Completeness [%] | 95.7 | 87 |
| Redundancy | 6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.3 | 285 | PEG 8000, PH 8.0 - 8.3 AT 4 - 12 DEGREES CELSIUS, temperature 285K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 |
