1IVS
CRYSTAL STRUCTURE OF THERMUS THERMOPHILUS VALYL-TRNA SYNTHETASE COMPLEXED WITH TRNA(VAL) AND VALYL-ADENYLATE ANALOGUE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-24 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.708 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 411.810, 411.810, 81.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.900 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.28100 * |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 22.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 150310 | |
| Completeness [%] | 96.5 | 87.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | Fukai, S., (2000) Cell (Cambridge,Mass.), 103, 793. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7-10 (mg/ml) | |
| 2 | 1 | drop | sodium cacodylate | 50 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 1.0 (M) | |
| 4 | 1 | drop | magnesium sulfate | 10 (mM) | |
| 5 | 1 | drop | 1,8-diaminooctane | 6 (%) | |
| 6 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 2.8 (M) | |
| 8 | 1 | reservoir | magnesium sulfate | 10 (mM) |
