1IVJ
Crystal Structure of Rat Hemeoxygenase-1 in Complex with Heme and Azide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.010, 65.010, 120.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dve |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.090 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.054 * | 0.325 * |
| Total number of observations | 169506 * | |
| Number of reflections | 23376 | |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 97.9 | 96.1 |
| Redundancy | 7.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | sodium formate, pottasium phosphate, sodium azide, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium formate | 4 (M) | |
| 2 | 1 | drop | protein | 18 (mg/ml) | |
| 3 | 1 | drop | potassium phosphate | 50 (mM) | pH7.0 |
| 4 | 1 | drop | sodium azide | 5 (mM) |
