1IV5
New Crystal Form of Human CD81 Large Extracellular Loop.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 64 |
| Unit cell lengths | 102.052, 102.052, 35.283 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.730 - 2.600 |
| R-factor | 0.23376 |
| Rwork | 0.229 |
| R-free | 0.28220 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.116 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.050 | 0.206 |
| Number of reflections | 32025 | |
| Completeness [%] | 98.2 | 89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | PEG 4000, DMSO, sodium chloride, sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
