1IUL
The structure of cell-free ID.343 from Thermus thermophilus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-13 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9747, 0.9800, 0.9804, 0.9824 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.098, 64.890, 34.971 |
| Unit cell angles | 90.00, 106.48, 90.00 |
Refinement procedure
| Resolution | 13.000 - 2.000 |
| R-factor | 0.199 |
| Rwork | 0.187 |
| R-free | 0.27000 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | 0.097 |
| Total number of observations | 31394 * | |
| Number of reflections | 8037 | |
| <I/σ(I)> | 21.8 | |
| Completeness [%] | 98.9 | 91.5 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.9 * | 293 * | SODIUM ACETATE, AMMONIUM SULFATE, PEG MME 2000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9.2 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 50 (mM) | pH4.9 |
| 3 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
| 4 | 1 | reservoir | PEG2000 MME | 15 (%) |
