1IUB
Fucose-specific lectin from Aleuria aurantia (Hg-derivative form)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-07-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00901, 1.00394, 0.918326 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 83.909, 83.909, 254.131 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 * - 2.310 |
| Rwork | 0.156 |
| R-free | 0.18300 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 25.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 200.000 * | 2.360 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rmerge | 0.067 | 0.290 |
| Number of reflections | 43457 | |
| <I/σ(I)> | 27.2 | |
| Completeness [%] | 95.4 | 93 |
| Redundancy | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 297 * | Fujihashi, M., (2003) Acta Crystallogr.,Sect.D, 59, 378. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | sodium phosphate | 8 (mM) | |
| 3 | 1 | reservoir | potassium phosphate | 1.5 (mM) | |
| 4 | 1 | reservoir | 137 (mM) | ||
| 5 | 1 | reservoir | 2.7 (mM) | pH7.2 | |
| 6 | 1 | reservoir | 1 (mM) |
