1IU4
Crystal Structure Analysis of the Microbial Transglutaminase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-02 |
| Detector | FUJI |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.410, 117.120, 85.740 |
| Unit cell angles | 90.00, 112.80, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.400 |
| R-factor | 0.2026 |
| Rwork | 0.199 |
| R-free | 0.26600 * |
| Structure solution method | MIR |
| RMSD bond length | 0.020 |
| RMSD bond angle | 3.655 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.040 | 0.130 |
| Total number of observations | 187008 * | |
| Number of reflections | 55351 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 1.0 | 92.1 * |
| Redundancy | 3.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 20 * | PEG1000, calcium chloride, cacodylate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG1000 | 25 (%(w/v)) | |
| 2 | 1 | reservoir | cacodylate-HCl | 100 (mM) | pH5.0 |
| 3 | 1 | reservoir | 25 (mM) | ||
| 4 | 1 | drop | protein | 15 (mg/ml) |
