1ISQ
Pyrococcus furiosus PCNA complexed with RFCL PIP-box peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 104 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 2001-02-17 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 63 |
| Unit cell lengths | 91.847, 91.847, 64.144 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| Rwork | 0.235 |
| R-free | 0.29130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ge8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 * | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.078 * | 0.345 * |
| Total number of observations | 60038 * | |
| Number of reflections | 13867 * | |
| <I/σ(I)> | 19.32 | 2.24 |
| Completeness [%] | 99.9 | 99.1 * |
| Redundancy | 4.4 * | 3.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | ammonium sulfate, sodium citrate, glycerol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | peptide | 1.8 (mM) | |
| 2 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.5 |
| 3 | 1 | reservoir | ammonium sulfate | 2.4 (M) | |
| 4 | 1 | reservoir | glycerol | 10 (%(v/v)) |
