1IS2
Crystal Structure of Peroxisomal Acyl-CoA Oxidase-II from Rat Liver
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.030, 91.501, 214.477 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.209 * |
| Rwork | 0.209 |
| R-free | 0.26000 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.283 |
| Total number of observations | 344046 * | |
| Number of reflections | 69647 | 6152 * |
| <I/σ(I)> | 16 | 2.1 |
| Completeness [%] | 94.7 | 85.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | PEG 20000, potassium phosphate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.0 (mg/ml) | |
| 2 | 1 | drop | PEG20000 | 3.0 (%(w/v)) | |
| 3 | 1 | drop | potassium phosphate | 20 (mM) | pH7.4 |
| 4 | 1 | reservoir | PEG20000 | 8-11 (%(w/v)) | |
| 5 | 1 | reservoir | potassium phosphate | 100 (mM) | pH7.4 |
