1IS1

Crystal structure of ribosome recycling factor from Vibrio parahaemolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	DIFFRACTOMETER
Detector	WEISSENBERG
Wavelength(s)	1.000
Spacegroup name	H 3 2
Unit cell lengths	84.799, 84.799, 142.727
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.200
Rwork	0.203
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005 *
RMSD bond angle	1.078 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.073	0.251 *
Total number of observations	82069 *
Number of reflections	10326
Completeness [%]	99.6	93.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	4 *	PEG 8000, sodium acetate, GDP, fusidic acid, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	MES/NaOH	50 (mM)	pH6.2
3	1	reservoir	sodium acetate	200 (mM)
4	1	reservoir	PEG8000	23-25 (%(w/v))