1IRI
Crystal structure of human autocrine motility factor complexed with an inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.651, 107.771, 270.345 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.400 |
| R-factor | 0.193 * |
| Rwork | 0.193 |
| R-free | 0.24100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1jiq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.528 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 * |
| Rmerge | 0.090 | 0.357 |
| Total number of observations | 550782 * | |
| Number of reflections | 92870 | |
| <I/σ(I)> | 7.3 | 2.1 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 5.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | cacodylate, sodium acetate, PEG8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | 0.05 (M) | ||
| 3 | 1 | drop | glycerol | 20 (%(v/v)) | |
| 4 | 1 | drop | Tris | 0.02 (M) | pH7.5 |
| 5 | 1 | reservoir | PEG8000 | 28 (%(w/v)) | |
| 6 | 1 | reservoir | sodium acetate | 0.2 (M) | |
| 7 | 1 | reservoir | glycerol | 20 (%(v/v)) | |
| 8 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
