1IR6

Crystal structure of exonuclease RecJ bound to manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-27
Detector	MARRESEARCH
Wavelength(s)	0.9795
Spacegroup name	P 21 3
Unit cell lengths	141.600, 141.600, 141.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.830 - 2.900
R-factor	0.228
Rwork	0.228
R-free	0.25900
Structure solution method	single-wavelength anomalous dispersion
RMSD bond length	0.008
RMSD bond angle	21.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000 *	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.049 *	0.279 *
Total number of observations	584147 *
Number of reflections	21198
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	20 *	ammonium sulfate, sodium/potassium tartrate, sodium citrate, glycerol, manganese chloride, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.0 (M)
2	1	reservoir	sodium potassium tartrate	0.2 (M)
3	1	reservoir	sodium citrate	50 (mM)
4	1	reservoir	glycerol	10 (%)	pH5.8
5	1	drop	protein	10 (mg/ml)
6	1	drop	Tris-HCl	20 (mM)
7	1	drop		100 (mM)
8	1	drop	EDTA	0.1 (mM)
9	1	drop	glycerol	10 (%)	pH7.5