1IR2
Crystal Structure of Activated Ribulose-1,5-bisphosphate Carboxylase/oxygenase (Rubisco) from Green alga, Chlamydomonas reinhardtii Complexed with 2-Carboxyarabinitol-1,5-bisphosphate (2-CABP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 100 |
Detector technology | DIFFRACTOMETER |
Collection date | 2000-06-09 |
Detector | WEISSENBERG |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 129.170, 174.750, 222.270 |
Unit cell angles | 90.00, 97.75, 90.00 |
Refinement procedure
Resolution | 39.890 - 1.840 |
R-factor | 0.152 * |
Rwork | 0.152 |
R-free | 0.18100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID: 1BUR |
RMSD bond length | 0.005 |
RMSD bond angle | 1.320 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.910 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.068 | 0.229 |
Total number of observations | 4698187 * | |
Number of reflections | 763078 | |
Completeness [%] | 91.0 | 72.7 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | PEG4000, HEPES-KOH, glycerol, NaHCO3, MgCl2, DTT, 2-carboxyarabinitol-1,5-bisphosphate (2-CABP), EDTA, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES-KOH | 80 (mM) | pH8.0 |
10 | 1 | reservoir | PEG4000 | 6-7 (%) | |
11 | 1 | reservoir | glycerol | 30 (%(v/v)) | |
2 | 1 | drop | EDTA | 1 (mM) | |
3 | 1 | drop | dithiothreitol | 5 (mM) | |
4 | 1 | drop | 20 (mM) | ||
5 | 1 | drop | 40 (mM) | ||
6 | 1 | drop | protein | 25 (mg/ml) | |
7 | 1 | reservoir | HEPES-KOH | 80 (mM) | pH8.0 |
8 | 1 | reservoir | EDTA | 1 (mM) | |
9 | 1 | reservoir | 20 (mM) |