1IQQ
Crystal Structure of Japanese pear S3-RNase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-25 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.650, 52.590, 47.570 |
| Unit cell angles | 90.00, 106.45, 90.00 |
Refinement procedure
| Resolution | 500.000 * - 1.500 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.20200 |
| Structure solution method | MIR |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.890 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.057 * | |
| Number of reflections | 33441 | |
| Completeness [%] | 96.3 | 90.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 * | Matsuura, T., (2001) Acta Crystallogr. Sect., D57, 172. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | MES/NaOH | 50 (mM) | pH7.0 |
| 3 | 1 | reservoir | MES-NaOH | 100 (mM) | pH7.0 |
| 4 | 1 | reservoir | sodium acetate | 200 (mM) | |
| 5 | 1 | reservoir | PEG6000 | 30 (%(w/v)) |
