1IQA

CRYSTAL STRUCTURE OF THE EXTRACELLULAR DOMAIN OF MOUSE RANK LIGAND

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL40B2
Synchrotron site	SPring-8
Beamline	BL40B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-12-16
Detector	RIGAKU RAXIS IV++
Wavelength(s)	0.9800
Spacegroup name	P 21 21 21
Unit cell lengths	65.680, 78.980, 100.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.200
R-factor	0.226 *
Rwork	0.226
R-free	0.27400
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	28.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.069	0.284
Total number of observations	257901 *
Number of reflections	26984
<I/σ(I)>	32	6.3
Completeness [%]	98.6	97.4
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	295	PEG8K, SODIUM CHLORIDE, ETHYLENE GLYCOL, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	25 (mM)	pH7.4
2	1	drop	protein	30 (mg/ml)
3	1	reservoir	PEG8000	14 (%(w/v))
4	1	reservoir	ethylene glycol	30 (%(v/v))
5	1	reservoir		300 (mM)
6	1	reservoir	HEPES	0.1 (M)	pH7.5