1IQA
CRYSTAL STRUCTURE OF THE EXTRACELLULAR DOMAIN OF MOUSE RANK LIGAND
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.680, 78.980, 100.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.200 |
| R-factor | 0.226 * |
| Rwork | 0.226 |
| R-free | 0.27400 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 28.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.069 | 0.284 |
| Total number of observations | 257901 * | |
| Number of reflections | 26984 | |
| <I/σ(I)> | 32 | 6.3 |
| Completeness [%] | 98.6 | 97.4 |
| Redundancy | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 295 | PEG8K, SODIUM CHLORIDE, ETHYLENE GLYCOL, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 25 (mM) | pH7.4 |
| 2 | 1 | drop | protein | 30 (mg/ml) | |
| 3 | 1 | reservoir | PEG8000 | 14 (%(w/v)) | |
| 4 | 1 | reservoir | ethylene glycol | 30 (%(v/v)) | |
| 5 | 1 | reservoir | 300 (mM) | ||
| 6 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
