1IQ0

THERMUS THERMOPHILUS ARGINYL-TRNA SYNTHETASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-06-05
Detector	RIGAKU RAXIS IV
Wavelength(s)	0.708
Spacegroup name	P 65
Unit cell lengths	154.747, 154.747, 84.573
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 * - 2.300
R-factor	0.215
Rwork	0.215
R-free	0.24200
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	1.160 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.051	0.186
Number of reflections	49236
<I/σ(I)>	35.8	7.4
Completeness [%]	95.8	94.3
Redundancy	7.94	6.54

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Shimada, A., (2001) Acta Crystallogr., D57, 272. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	100 (mM)	pH7.0
2	1	drop	PEG8000	10 (%(v/v))
3	1	drop	ethylene glycol	8 (%(v/v))
4	1	drop	polyvinylpyrrolidone15000	2.5 (%)
5	1	reservoir	ethylene glycol	8 (%(v/v))