1IPK

CRYSTAL STRUCTURES OF RECOMBINANT AND NATIVE SOYBEAN BETA-CONGLYCININ BETA HOMOTRIMERS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1996-10-28
Detector	SIEMENS HI-STAR
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	80.510, 63.480, 131.430
Unit cell angles	90.00, 90.01, 90.00

Refinement procedure

Resolution	7.000 - 2.800 *
R-factor	0.205 *
Rwork	0.205
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005 *
RMSD bond angle	1.271 *
Data reduction software	SAINT
Data scaling software	SAINT

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.055 *
Total number of observations	101661 *
Number of reflections	33007
Completeness [%]	91.0
Redundancy	3.08

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8 *	4 *	PEG10000, MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
10	1	reservoir	EDTA	1 (mM)
11	1	reservoir	p-APMSF	0.1 (mM)
12	1	reservoir		0.02 (%)
2	1	drop	citrate	50 (mM)	pH4.8
3	1	drop		0.4 (M)
4	1	drop	EDTA	1 (mM)
5	1	drop	p-APMSF	0.1 (mM)
6	1	drop		0.02 (%)
7	1	reservoir	PEG6000	5 (%)
8	1	reservoir	citrate	50 (mM)	pH4.8
9	1	reservoir		0.4 (M)