1IPB

CRYSTAL STRUCTURE OF EUKARYOTIC INITIATION FACTOR 4E COMPLEXED WITH 7-METHYL GPPPA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL24XU
Synchrotron site	SPring-8
Beamline	BL24XU
Spacegroup name	P 43
Unit cell lengths	89.260, 89.260, 38.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.196 *
Rwork	0.196
R-free	0.22900
RMSD bond length	0.006 *
RMSD bond angle	1.280 *
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	1.900 *
Rmerge	0.084 *
Number of reflections	20089 *
Completeness [%]	82.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	15 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES-KOH	100 (mM)	pH7.5
2	1	reservoir	PEG6000	27 (%(w/v))
3	1	reservoir	ammonium sulfate	0.2 (M)