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CRYSTAL STRUCTURE OF NICOTIANA ALATA GEMETOPHYTIC SELF-INCOMPATIBILITY ASSOCIATED SF11-RNASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-04-12
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.02
Spacegroup name	P 1 21 1
Unit cell lengths	65.869, 44.731, 64.360
Unit cell angles	90.00, 90.27, 90.00

Refinement procedure

Resolution	65.870 - 1.550
R-factor	0.185 *
Rwork	0.185
R-free	0.21500
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	22.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.940	1.610
High resolution limit [Å]	1.550	1.550
Rmerge	0.061	0.307
Total number of observations	237209 *
Number of reflections	53199
<I/σ(I)>	14.4
Completeness [%]	97.4	95.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	293	Ida, K., (2001) Acta Crystallog., D57, 143. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	cacodylate	100 (mM)	pH6.5
3	1	reservoir	PEG6000	28-34 (%(w/w))
4	1	reservoir		0.8 (M)