1IOK
CRYSTAL STRUCTURE OF CHAPERONIN-60 FROM PARACOCCUS DENITRIFICANS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-21 |
| Detector | FUJI |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 286.355, 286.355, 153.463 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 3.200 |
| R-factor | 0.2035 |
| Rwork | 0.204 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 0.881 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.073 | 0.464 |
| Total number of observations | 582211 * | |
| Number of reflections | 103358 * | 10279 * |
| <I/σ(I)> | 20.6 | 3.33 |
| Completeness [%] | 98.5 | 99.3 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 20 * | PEG 6000, LiCl, Bicine-Na, pH 9.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 20 * | PEG 6000, LiCl, Bicine-Na, pH 9.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 60 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 4.0 (%(w/v)) | |
| 3 | 1 | reservoir | 1.0 (M) | ||
| 4 | 1 | reservoir | Bicine-Na | 100 (mM) |
