1IO4
CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA RUNT DOMAIN-CBFBETA CORE DOMAIN HETERODIMER AND C/EBPBETA BZIP HOMODIMER BOUND TO A DNA FRAGMENT FROM THE CSF-1R PROMOTER
Experimental procedure
| Experimental method | THREE WAVELENGTH FROM GOLD DERIVATIVE |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-07 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 121.108, 163.600, 109.326 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 3.000 |
| R-factor | 0.247 * |
| Rwork | 0.247 |
| R-free | 0.29900 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.9) |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.062 | 0.372 |
| Total number of observations | 66989 * | |
| Number of reflections | 21113 | |
| <I/σ(I)> | 15.5 | 1.7 |
| Completeness [%] | 97.1 | 90.8 |
| Redundancy | 3.173 | 3.246 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 297 | Tahirov, T.H., (2001) Acta Crystallogr., D57, 850. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCl | ||
| 10 | 1 | 2 | MPD | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | glycerol | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | DTT | ||
| 6 | 1 | 1 | PEG 8000 | ||
| 7 | 1 | 1 | MES | ||
| 8 | 1 | 2 | KCl | ||
| 9 | 1 | 2 | MgCl2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein-DNA solution | 10 (mg/ml) | |
| 2 | 1 | reservoir | 0.1 (M) | ||
| 3 | 1 | reservoir | 0.01 (M) | ||
| 4 | 1 | reservoir | PEG400 | 10 (%(v/v)) | |
| 5 | 1 | reservoir | MES | 0.05 (M) |
