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HOW THE ANTI-(METAL CHELATE) ANTIBODY CHA255 IS SPECIFIC FOR THE METAL ION OF ITS ANTIGEN: X-RAY STRUCTURES FOR TWO FAB'(SLASH)HAPTEN COMPLEXES WITH DIFFERENT METALS IN THE CHELATE

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	99.300, 84.600, 76.700
Unit cell angles	90.00, 136.20, 90.00

Refinement procedure

Resolution	7.000 - 2.200
R-factor	0.188
Rwork	0.188
RMSD bond length	0.017
RMSD bond angle	2.600
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.200 *	2.200 *
Rmerge	0.094 *
Number of reflections	22000 *
Completeness [%]	99.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	PEG4000	10 (%)
3	1	drop		0.1 (M)
4	1	drop	HEPES	50 (mM)
5	1	reservoir			double the amount of the drop(except for protein)