1IMX
1.8 Angstrom crystal structure of IGF-1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9197, 1.5406, 0.9199, 0.8610 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 31.831, 71.055, 65.996 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.000 - 1.820 |
| R-factor | 0.245 * |
| Rwork | 0.245 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.054 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNX (2000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.830 |
| High resolution limit [Å] | 1.820 | 1.800 |
| Rmerge | 0.038 | 0.290 |
| Total number of observations | 44573 * | |
| Number of reflections | 6871 | |
| <I/σ(I)> | 16.7 | 3.5 |
| Completeness [%] | 98.2 * | 99.1 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.5 * | 293 | PEG 3350, deoxy big CHAPS, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 0.15 (M) | ||
| 2 | 1 | drop | sodium acetate | 20 (mM) | |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | reservoir | PEG3350 | 24 (%) | |
| 5 | 1 | reservoir | sodium cacodylate | 0.1 (M) |
