1IM3

Crystal Structure of the human cytomegalovirus protein US2 bound to the MHC class I molecule HLA-A2/tax

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2000-06-27
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	95.480, 96.700, 99.540
Unit cell angles	109.02, 109.46, 107.93

Refinement procedure

Resolution	38.850 - 2.200
R-factor	0.206
Rwork	0.206
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1duz
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.045	0.287
Total number of observations	139486 *
Number of reflections	13849 *
<I/σ(I)>	14.2	3.3
Completeness [%]	98.0	97.1
Redundancy	2.1	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	18 *	PEG 6000, MPD, Tris, pH 7.75, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris	10 (mM)
3	1	drop		150 (mM)
4	1	reservoir	PEG6000	12-14 (%)
5	1	reservoir	MPD	7 (%)
6	1	reservoir	Tris	100 (mM)