1IKO

CRYSTAL STRUCTURE OF THE MURINE EPHRIN-B2 ECTODOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-02
Detector	SBC-2
Wavelength(s)	1.033
Spacegroup name	P 65 2 2
Unit cell lengths	59.283, 59.283, 168.868
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.410 - 1.920
R-factor	0.215 *
Rwork	0.216
R-free	0.24800 *
Structure solution method	SIRAS
RMSD bond length	0.010 *
RMSD bond angle	1.600 *
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.500 *	1.990
High resolution limit [Å]	1.920	1.920
Rmerge	0.042 *	0.219 *
Total number of observations	315392 *
Number of reflections	14283 *
<I/σ(I)>	48	18
Completeness [%]	99.5	99.8
Redundancy	22	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	4 *	magnesium chloride, PEG 4000, trishydroxyethylmethane, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30-98 (mg/ml)
2	1	reservoir	Tris	100 (mM)	pH8.5
3	1	reservoir		200 (mM)
4	1	reservoir	PEG4000	20 (%)