1IK9
CRYSTAL STRUCTURE OF A XRCC4-DNA LIGASE IV COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.062, 79.628, 242.142 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.260 - 2.300 |
| R-factor | 0.228 * |
| Rwork | 0.228 |
| R-free | 0.26600 |
| Structure solution method | SAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.260 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.051 | 0.229 |
| Total number of observations | 72477 * | |
| Number of reflections | 34224 | |
| <I/σ(I)> | 17.9 | 2.3 |
| Completeness [%] | 85.5 | 44.4 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | used seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.050 (mM) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | PEG6000 | 1.5 (%(w/v)) | |
| 5 | 1 | drop | MES | 0.05 (M) | pH6.0 |
| 7 | 1 | drop | xylitol precipitant solution | 1.5 (%(w/v)) | |
| 8 | 1 | reservoir | PEG6000 | 6 (%(w/v)) | |
| 9 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |
