1IK3
LIPOXYGENASE-3 (SOYBEAN) COMPLEX WITH 13(S)-HYDROPEROXY-9(Z),11(E)-OCTADECADIENOIC ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 296 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.650, 137.280, 61.890 |
| Unit cell angles | 90.00, 95.56, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lnh PROTEIN ONLY |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.064 | 0.352 |
| Total number of observations | 107634 * | |
| Number of reflections | 41035 | |
| <I/σ(I)> | 11.5 | 1.3 |
| Completeness [%] | 78.0 | 68 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 23 * | drop1:drop2:drop3:drop4=3:6:1:2ratio, Skrzypczak-Jankun, E., (1997) Proteins: Struct.,Funct., Genet., 29, 15. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop1 | L3 | 10 (mg/ml) | |
| 2 | 1 | drop1 | Tris-HCl | 0.1 (M) | pH7.0 |
| 3 | 1 | drop2 | PEG8000 | 20 (%(w/v)) | |
| 4 | 1 | drop2 | sodium citrate phosphate | 0.05 (M) | pH4.6 |
| 5 | 1 | drop2 | 0.2 (%(w/v)) | ||
| 6 | 1 | drop3 | sodium phosphate | 0.1 (M) | pH7.0 |
| 7 | 1 | drop4 | water | ||
| 8 | 1 | reservoir | PEG8000 | 20 (%) |
