1IJD
Crystallographic Structure of the LH3 Complex from Rhodopseudomonas acidophila strain 7050
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-08-29 |
Detector | MARRESEARCH |
Wavelength(s) | 0.87 |
Spacegroup name | H 3 2 |
Unit cell lengths | 117.260, 117.260, 295.920 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.000 - 3.000 |
R-factor | 0.243 * |
Rwork | 0.243 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1KZU (LH2 complex from Rps. acidophila strain 10050) |
RMSD bond length | 0.013 * |
RMSD bond angle | 0.021 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | AMoRE |
Refinement software | RESTRAIN |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.000 | 2.870 |
High resolution limit [Å] | 3.000 * | 2.800 |
Rmerge | 0.085 * | 0.370 * |
Number of reflections | 18970 | |
<I/σ(I)> | 4.6 | 1.3 |
Completeness [%] | 97.0 * | 96.4 * |
Redundancy | 3.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.2 | 291 | McLuskey, K., (1999) Acta Crystallogr., Sect.D, 55, 885. * |
1 | VAPOR DIFFUSION, SITTING DROP | 9.2 | 291 | McLuskey, K., (1999) Acta Crystallogr., Sect.D, 55, 885. * |
1 | VAPOR DIFFUSION, SITTING DROP | 9.2 | 291 | McLuskey, K., (1999) Acta Crystallogr., Sect.D, 55, 885. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | potassium phosphate | 4.0 (M) | pH9.2 |
2 | 1 | drop | benzamidine hydrochloride | 2.5 (%(w/v)) | |
3 | 1 | reservoir | ammonium sulfate | 2.3 (M) | pH9.7 |