1IIL
CRYSTAL STRUCTURE OF PRO253ARG APERT MUTANT FGF RECEPTOR 2 (FGFR2) IN COMPLEX WITH FGF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97624 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.796, 72.490, 89.919 |
| Unit cell angles | 90.01, 89.82, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ev2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.041 | 0.137 |
| Total number of observations | 321836 * | |
| Number of reflections | 85442 * | |
| <I/σ(I)> | 26.1 | |
| Completeness [%] | 97.8 | 95.9 |
| Redundancy | 3.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 298 | PEG 4000, Isopropanol, HEPES-NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES-NaOH | 25 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | PEG4000 | 10-15 (%) | |
| 5 | 1 | reservoir | isopropanol alcohol | 10 (%) | |
| 6 | 1 | reservoir | HEPES-NaOH | 0.1 (M) |
