1IIC

Crystal Structure of Saccharomyces cerevisiae N-myristoyltransferase with Bound MyristoylCoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	2000-04-10
Detector	RIGAKU RAXIS IV
Spacegroup name	P 2 2 21
Unit cell lengths	75.129, 97.060, 141.809
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.220 - 2.200
R-factor	0.236
Rwork	0.236
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nmt
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.048 *
Total number of observations	341253 *
Number of reflections	48952
<I/σ(I)>	19.4	7
Completeness [%]	91.7	79.59

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	20 *	PEG 4000, ammonium acetate, sodium cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	reservoir	ammonium acetate	0.1 (M)
3	1	reservoir	sodium cacodylate	0.1 (M)	pH6.4
4	1	reservoir	PEG4000	20 (%)