1II7
Crystal structure of P. furiosus Mre11 with manganese and dAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-07-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98400 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.290, 88.740, 145.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.046 | 0.326 |
| Total number of observations | 431781 * | |
| Number of reflections | 48885 * | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 98.1 | 86.1 |
| Redundancy | 8.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 4 * | 100 mM Ches, 400 mM ammonium sulfate, 35% OEG 6K, 1 mM manganese, 10 mM dAMP, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | phosphate | 20 (mM) | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | EDTA | 0.1 (mM) | |
| 5 | 1 | drop | glycerol | 5 (%) | |
| 6 | 1 | reservoir | ches | 100 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 400 (mM) | |
| 8 | 1 | reservoir | PEG6000 | 35 (%) |
