1IHX
Crystal structure of two D-glyceraldehyde-3-phosphate dehydrogenase complexes: a case of asymmetry
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 153.409, 100.506, 128.440 |
Unit cell angles | 90.00, 110.49, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.800 |
R-factor | 0.177 |
Rwork | 0.177 |
R-free | 0.20500 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.410 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.126 | 0.379 * |
Total number of observations | 119278 * | |
Number of reflections | 44055 | |
<I/σ(I)> | 10 | 3 |
Completeness [%] | 98.0 | 97.9 |
Redundancy | 5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 298 | Shen, Y.O., (2000) Chin. Sci. Bull., 45, 1199. * |