1IHG
Bovine Cyclophilin 40, monoclinic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.125, 47.328, 85.563 |
| Unit cell angles | 90.00, 119.42, 90.00 |
Refinement procedure
| Resolution | 24.000 - 1.800 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CYCLOPHILIN A |
| RMSD bond length | 0.070 * |
| RMSD bond angle | 0.230 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.096 |
| Number of reflections | 41259 | |
| <I/σ(I)> | 20.67 | 9.03 |
| Completeness [%] | 98.3 | 87.3 |
| Redundancy | 3.7 | 1.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | PEG 4000, MES Glycerol Sodium Chloride, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 50 (%) | 0.320ml |
| 2 | 1 | reservoir | MES | 1 (M) | 0.100ml |
| 3 | 1 | reservoir | glycerol | 50 (%(v/v)) | 0.200ml |
| 4 | 1 | reservoir | 4 (M) | 0.100 | |
| 5 | 1 | reservoir | water | 0.280ml | |
| 6 | 1 | drop | protein | 10-80 (mg/ml) | |
| 7 | 1 | drop | Tris | 20 (mM) | |
| 8 | 1 | drop | 100 (mM) |
