1IHB

CRYSTAL STRUCTURE OF P18-INK4C(INK6)

Experimental procedure

Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	1996-08-18
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	55.510, 151.700, 40.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.950
R-factor	0.205 *
Rwork	0.209
R-free	0.28400 *
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	22.100 *
Data reduction software	DENZO
Phasing software	PHASES
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	1.950
Rmerge	0.042 *
Number of reflections	24019
Completeness [%]	95.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	4 *	VAPOR DIFFUSION, 4 DEG. C, 2 MICROLITER HANGING DROP CONTAINING 5 MG/ML PROTEIN, 20 MM TRIS (PH 8.5), 25 MM (NH4)2 HPO4, 0.5 MM DDT, 7% PEG 6K, 1 MM NACL EQUILIBRATED OVER A RESERVOIR CONTAINING 14% PEG 6K AND 2 M NACL. WITHIN 8 DAYS, 0.2 X 0.2 X 0.4 MM CRYSTALS., vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		25 (mM)
4	1	drop	dithiothreitol	0.5 (mM)
5	1	drop	PEG6000	7 (%)
6	1	drop		1 (M)
7	1	reservoir	PEG6000	14 (%)
8	1	reservoir		2 (M)