1IGS
INDOLE-3-GLYCEROLPHOSPHATE SYNTHASE FROM SULFOLOBUS SOLFATARICUS AT 2.0 A RESOLUTION
Experimental procedure
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-12-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.000, 73.800, 104.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.073 |
| Total number of observations | 205248 * |
| Number of reflections | 29607 |
| Completeness [%] | 96.0 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 | 4 * | pH 5. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | potassium phosphate | 50 (mM) | |
| 2 | 1 | drop | EDTA | 5 (mM) | |
| 3 | 1 | drop | DTE | 2 (mM) | |
| 4 | 1 | drop | protein | 20 (mg/ml) | |
| 5 | 1 | reservoir | ammonium sulfate | 26-30 (%sat) |
