1IG8

Crystal Structure of Yeast Hexokinase PII with the correct amino acid sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE D03B-MX1
Synchrotron site	LNLS
Beamline	D03B-MX1
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1998-09-10
Detector	MARRESEARCH
Wavelength(s)	1.38
Spacegroup name	I 4
Unit cell lengths	142.809, 142.809, 58.462
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.000 - 2.200
Rwork	0.162
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	P152K mutant of yeast hexokinase
RMSD bond length	0.011
RMSD bond angle	0.033
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.000	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.113 *	0.420
Number of reflections	60395
Completeness [%]	95.1	96.6
Redundancy	2.12	1.82

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5	298	2.3-3.2M Ammonium sulphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.6-2.4 (M)
2	1	reservoir	potassium phosphate	100 (mM)