1IFV

CRYSTAL STRUCTURE OF PATHOGENESIS-RELATED PROTEIN LLPR10.1B FROM YELLOW LUPINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	292
Detector technology	IMAGE PLATE
Collection date	1999-11-09
Detector	MARRESEARCH
Wavelength(s)	1.54178
Spacegroup name	P 21 21 2
Unit cell lengths	64.880, 107.445, 46.879
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.250
R-factor	0.186 *
Rwork	0.186
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	LlPR10.1A
RMSD bond length	0.015
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.102	0.399
Total number of observations	88882 *
Number of reflections	15594 *
<I/σ(I)>	13.26	2.78
Completeness [%]	97.2	94.7
Redundancy	5.6 *	3.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8 *	292	sodium citrate, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	5.6 (mg/ml)
2	1	1	sodium citrate	0.9 (M)
3	1	1	HEPES	0.1 (M)	pH8.0