1IF1
INTERFERON REGULATORY FACTOR 1 (IRF-1) COMPLEX WITH DNA
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 111 |
| Detector technology | CCD |
| Collection date | 1996-06 |
| Detector | CUSTOM-MADE |
| Spacegroup name | H 3 |
| Unit cell lengths | 84.800, 84.800, 203.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 3.000 |
| R-factor | 0.242 |
| Rwork | 0.242 |
| R-free | 0.30900 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.230 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.061 | 0.090 |
| Total number of observations | 125805 * | |
| Number of reflections | 21657 * | |
| <I/σ(I)> | 15.3 | 13.5 |
| Completeness [%] | 99.1 | 99.7 |
| Redundancy | 4.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | Escalante, C.R., (1997) FEBS Lett., 414, 219. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 18-22 (%) | |
| 2 | 1 | 1 | ammonium acetate | 300 (mM) |
