1IEJ

OVOTRANSFERRIN, N-TERMINAL LOBE, HOLO FORM, AT 1.65 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1996-09-12
Detector	SIEMENS HI-STAR
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	46.460, 85.950, 76.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.650
R-factor	0.173 *
Rwork	0.173
R-free	0.24200
RMSD bond length	0.012
RMSD bond angle	24.200 *
Data reduction software	SAINT
Data scaling software	SAINT
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.620
Rmerge	0.058
Total number of observations	135942 *
Number of reflections	35322
Completeness [%]	89.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.9	293	used to seed, Dewan, J.C., (1993) Biochemistry, 32, 11963. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	59 (mg/ml)
2	1	drop	acetate	0.02 (M)
3	1	drop		0.01 (%)
4	1	drop	PEG6000	4-5 (%)
5	1	drop	acetate	0.02 (M)
6	1	reservoir	PEG6000	4-5 (%)
7	1	reservoir	acetate	0.02 (M)