1IDM

3-ISOPROPYLMALATE DEHYDROGENASE, LOOP-DELETED CHIMERA

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-03-26
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	78.450, 78.450, 158.070
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	5.000 - 2.200
R-factor	0.17
Rwork	0.170
RMSD bond length	0.010
RMSD bond angle	1.410 *
Data reduction software	PROCESS
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.200 *
Rmerge	0.038
Total number of observations	44526 *
Number of reflections	27190
Completeness [%]	76.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6 *		Sakurai, M., (1992) J.Biochem., 112, 173. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	potassium phosphate	20 (mM)
3	1	reservoir	ammonium sulfate	1.0-2.0 (M)
4	1	reservoir	potassium phosphate	20 (mM)