1IDC

ISOCITRATE DEHYDROGENASE FROM E.COLI (MUTANT K230M), STEADY-STATE INTERMEDIATE COMPLEX DETERMINED BY LAUE CRYSTALLOGRAPHY

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Detector technology	IMAGE PLATE
Collection date	1993-10
Detector	FUJI
Wavelength(s)	0.7 - 2.1
Spacegroup name	P 43 21 2
Unit cell lengths	105.100, 105.100, 150.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.500
R-factor	0.169
Rwork	0.169
RMSD bond length	0.018
RMSD bond angle	25.070 *
Data reduction software	OXFORD (LAUE PACKAGE (J.CAMPBELL))
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.500 *	2.500 *
Rmerge	0.089
Number of reflections	17316
Completeness [%]	62.0	82 *
Redundancy	18.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	dephosphorylated IDH	28 (mg/ml)
2	1	reservoir	ammonium sulfate	34 (%sat)
3	1	reservoir		100 (mM)
4	1	reservoir		35 (mM)
5	1	reservoir	citric acid	9 (mM)
6	1	reservoir	dithiothreitol	0.2 (mM)